Olympus IX71 Instrukcja Użytkownika

INSTRUCTIONSIX71/IX51INVERTED RESEARCH MICROSCOPE/INVERTED BASIC MICROSCOPEA X 7 3 1 9This instruction manual is for the Olympus Inverted Microscopes

6}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 55 to 65). · The illustration shows the system composed of modules e

7IX71/IX51IX51Filter holder (Page 26)Aperture iris diaphragmlever (Page 25)Diopter adjustmentring (Page 20)Indicator plate pocketCoarse adjustment kno

8Left Side View of Microscope FrameIX71IX51Allen screwdriverstorage position (Rear panel)Side portTransport lock knob#Be sure to remove this before us

9IX71/IX51Other ModulesMonocular TubeU-MO10X eyepiece(Fixed)Trinocular Observation Tube HU-TR30H-2Straight photo tube mount (Page 44)Light path select

10Long WD Universal CondenserIX2-LWUCD}This is a manually controlled condenser.Centering knob insertion holes(x 2)ULWCD CondenserIX-ULWCD}This is a ma

11IX71/IX51Power Supply UnitTH4}The applicable halogen bulb is the 12V100WHAL-L.For details, refer to the separate instruction manual.Light intensity

12TRANSMITTED LIGHT BRIGHTFIELDOBSERVATION PROCEDURE}The following flow shows the operating procedure for the transmitted light brightfield observatio

13IX71/IX51With the IX71Power Supply UnitTH4Hand SwitchTH4-HSWith the IX51When the TL4 powersupply unit is used} Make a photocopy of the observation p

14}The power supply unit to be used is variable depending on the illumination column in use. · IX2-ILL100: TH4 power supply unit. · IX2-ILL30: TL4 pow

15IX71/IX51When the TH4-HS Hand Switch is Used (Fig. 7)}The illumination brightness can be adjusted from the hand switch in thesame way as on the IX71

164 Frame Clamping Plate}This is the module for clamping the microscope frame onto ananti-vibration platform. The applicable anti-vibration platfor

17IX71/IX513 Detaching the Fine Adjustment Knob(Fig. 11)}The fine adjustment knob is designed detachable in order to preventinterference between th

18Fig. 16Fig. 154-3 Stage1 Placing the Specimen(Figs. 13 to 16)With the IX2-SFR or IX-SVL2 Stage (Fig. 13)Place the specimen on the center of the

19IX71/IX512 Moving the Specimen(Fig. 17)With the IX2-SFR or IX-SVL2 StageTo move the specimen to a desired position, rotate the X-axis knob @and Y

20While looking through the eyepieces, adjust the binocular vision until theleft and right fields of view coincide completely. The index dot · indicat

21IX71/IX51Eyepiece micrometer disks can be inserted into the WHN10X-H (orWHN10X) eyepieces.Use 24 mm dia. x 1.5 mm micrometer disks.Following Fig. 24

227 Adjusting the Tilt (U-TBI90 Only)(Fig. 27)}Adjust the height and tilt of the observation tube to obtain the mostcomfortable viewing position.Ho

23IX71/IX512 Mounting the Filters(Figs. 29 to 31)}45 mm diameter, maximum 6 mm thick filters can be mounted. Variousfilters, such as the provided f

24Fig. 34Fig. 35}The field iris diaphragm is used to adjust the diameter of the illuminationbeam in accordance with the objective in use. Adjust the d

25IX71/IX51Fig. 364-6 Illumination Column IX2-ILL30}This illumination column is provided with a condenser as standard. The applicable objectives rang

IX71/IX51CONTENTS56-1112, 1314-31IMPORTANT — Be sure to read this section for safe use of the equipment. —1 MODULE NOMENCLATURE2 CONTROLS34

263 Mounting the Filters(Figs. 38 & 39)}Wait until the filters have cooled down enough before replacing a filter.Take out the filter holder @ a

27IX71/IX51With the IX2-LWUCD/IX2-LWUCDA2 Universal Condenser orIX-ULWCD Condenser1. Rotate the turret @ (either manually or electrically) to select t

28With the U-UCD8 Universal Condenser (Fig. 41)#For operation of the U-UCD8, refer to the instruction manual providedwith it.Since this condenser is u

29IX71/IX51Fig. 42}In general, the potential resolving power of an objective is fully utilized if thediaphragm is stopped down to correspond with the

301 Oil-Proof Cap (UIS Series only)(Fig. 44)}By mounting the oil-proof cap (Type C1 or Type C2) on the tip of theapplicable objective, you can prev

31IX71/IX511. Correction is possible according to the vessel bottom thickness. (Seepage 53,54 when the correction collar objective is used.)When the t

32}A phase contrast objective, phase contrast optical element and the U-CT30 centering telescope (which is not requiredwhen the U-BI90CT binocular obs

33IX71/IX512(Figs. 46 & 47)}For the U-UCD8, refer to the separate instruction manual.}Do not engage any optical element in the BF (brightfield) li

344. Hold a phase contrast ring and, while pushing the spring ƒ inside theturret with the edge of the phase contrast ring …, insert the ring com-plete

35IX71/IX51}Before proceeding to the following, open the aperture iris diaphragmbecause flare would be observed at the center when it is stopped down.

7 TROUBLESHOOTING GUIDE8 SPECIFICATIONS95 OTHER OBSERVATION METHODS5-1 Phase Contrast Observation (Using the IX2-ILL100 Column) ...

365-2 Phase Contrast Observation (Using the IX2-ILL30 Column)}A phase contrast slider, phase contrast objective, phase contrast optical element and t

37IX71/IX511. Attach the phase contrast slider @ to the illumination column so that theslider’s indication surface faces upwards and the finger hook p

38}If a plastic petri dish is used, the normal optical performance of DIC observation cannot be manifested due to thepolarization characteristic of th

39IX71/IX51CPlanFl 10X (IX-DP10) (IX-DP10) ––– –––LCPlanFl 20X (IX-DP20) (IX-DP20) (IX-DP20HC) (IX-DP20HR)40X IX-DP40 IX-DP40 IX-DP40HC I

402 Attaching the DIC Optical Elements(Fig. 59)}The attaching method is identical to that for the phase contrast opticalelements (pages 33-34), exc

41IX71/IX514 Attaching the Polarizer (IX-LWPO)(Fig. 63)#The polarizer can be mounted only on the IX2-LWUCD or IX2-LWUCDA2.}This polarizer has been

421. Rotate the condenser turret to engage the suitable optical element forthe objective in use in the light path.2. Engage the objective to be used i

43IX71/IX51}An analyzer and polarizer are required for simplified polarized light observation. The applicable condenser is the IX2-LWUCD or IX2-LWUCDA

446-1 PhotomicrographyPHOTOMICROGRAPHY AND TV OBSERVATION}Use the U-TR30H-2 trinocular observation tube or the side port for photomicrography.Photomi

45IX71/IX513 Attaching the Photomicrographic System(Fig. 71) · Attach the photomicrographic system directly onto the photomicrographicsystem mount

1IX71/IX51IMPORTANTThis microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used withmodules designed for the IX2

466 Adjusting the IlluminationCorrect illumination is more crucial for photomicrography than for observation because flawless pictures cannot beobt

47IX71/IX516-2 TV Observation}The following five methods are available for TV observation.1. Attaching the video camera onto the side port2. Attachin

483 Attaching the Camera on the Monocular Tube(Fig. 75)1. Using the Allen screwdriver provided with the microscope frame, loosenthe clamping screw

49IX71/IX51Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problemsoccur, please r

50Problem Cause Remedy PageMagnification selector knob is in anintermediate position.Engage the knob all the way in or at aclick stop according to the

51IX71/IX51Problem Cause Remedy Page–g) Image is blurred. Objective is engaged incorrectly in thelight path.Make sure that revolving nosepiececlicks i

52Light path selectionS8F-3: Binocular 100% or Binocular 20%/Side port 80%(with shockless mechanism)S1F-3: Binocular 100%/Side port 100%Intermediate m

53IX71/IX51ObjectivesSpecificationsUIS2SeriesPlan achromatfor phasecontrastobservationCPlanN10XPh NA 0.25, WD 10.0 mm, ring slit IX-PHC or PHCUCPlanFL

54Module SpecificationOperating environment · Indoor use · Altitude: Max. 2000 m · Ambient temperature: 5° to 40°C (41° to 104°F) · Maximum relative

55IX71/IX51ASSEMBLY9-1 Assembly DiagramThe diagram below shows the sequence of assembly of the modules. The numbers indicate the order of assembly.Th

2Fig. 21. After the equipment has been used in an observation of a specimen thatis accompanied with a potential of infection, clean the parts coming i

569-2 Detailed Assembly ProceduresSystem Using the IX2-ILL100 Illumination Column1. Aligning the two guide holes on the illumination column with the

57IX71/IX51System Using the IX2-ILL30 Illumination Column}The IX2-ILL30 illumination column can be attached in the same manneras the IX2-ILL100.2 A

584 Attaching the Stage(Figs. 83 to 85)}The following stage mounting procedures are commonly applicable tothe IX2-SFR, IX-SVL2, IX2-SP and IX2-KSP.

59IX71/IX51<< Attaching the Scales >>}The IX-MVR mechanical stage is provided with the scales for use with a96-well microtiter plate.1. Lo

60Fig. 91@Fig. 92@Fig. 93@5 Attaching the Objectives(Fig. 91)Remove the stage center plate and attach the objectives to the revolvingnosepiece thro

61IX71/IX51Fig. 94@Fig. 95²³Universal Condenser (U-UCD8) (Figs. 94 & 95)<< Attaching onto the UCD Adapter >>1. Using the Allen screwdr

62Fig. 96@²Fig. 97Fig. 98@Fig. 99²7 Attaching the Observation Tube(Figs. 96 to 99)U-BI90CT, U-BI90 or U-TBI90 Binocular Tube(U-MO Monocular Tube)1.

63IX71/IX51Fig. 100@Fig. 101@8 Attaching the Eyepieces(Fig. 100)1. Remove the eyepieces’ dust caps.2. Insert the WHN10X-H eyepiece with helicoid in

64Fig. 102@²³|Fig. 103@²ƒ…Fig. 104†‡9 Connecting the Cables(Figs. 102 to 104)Cables and cords are vulnerable when bent or twisted. Never subjectthe

65IX71/IX51Fig. 105@²³|Fig. 106@@²10(Figs. 105 & 106)Rubber foot seats}The microscope frame clamping method is variable depending onwhether the fr

3IX71/IX511. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.2. Do not use the microsco

66Table 1 Certified CordA power supply cord should be certified by one of the agencies listed in Table 1, or comprised of cordage marked with an agen

67IX71/IX51Table 2 HAR Flexible CordAPPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODSApproval OrganizationPrinted or Embossed Harmoniz

68{Study the instruction manual for the lamp housing before inspection.{For safe use of the lamp housing, we recommend performing the following inspec

MEMO

MEMO

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, JapanWendenstraße 14-18, 20097 Hamburg, Germany3500 Corporate Parkway, P.O. Box 61

46. Restrictions in TV observation@The following combinations are not permitted in consideration of the optical performance. · IX2-SPT + PE4X + U-PMTV

5IX71/IX51MODULE NOMENCLATURE}The modules shown below are only the representative modules. As there are other modules which can be combinedwith the mi